Kinglassie is a parish, which lies in Fife Council Area, some 2 miles (4 km) southwest of Glenrothes and 4 miles (7 km) northwest of Kirkcaldy in Fife.
It is located in the old county of Fife, which disappeared following the Local Government (Scotland) Act 1974.
The parish has an area of 34.1 sq. km (13.1 sq. miles). Kinglassie has 5 neighbouring parishes; namely Auchterderran, Kirkcaldy & Dysart, Leslie, Markinch and Portmoak. The boundaries of this parish were modified among significant changes recommended by the Boundary Commissioners after the Local Government (Scotland) Act 1889.